Third fastest in the opening session of the weekend was Mugello race winner Jorge Lorenzo (Ducati Team), just 0.001 behind his teammate, with Marc Marquez (Repsol Honda Team) fourth fastest – 0.426 behind Rossi.
Andrea Iannone (Team Suzuki Ecstar), who finished ninth in FP1, crashed at Turn 10 early in the session. Franco Morbidelli (EG 0,0 Marc VDS) was another faller, with Karel Abraham (Angel Nieto Team) also crashing – riders ok.
Jack Miller (Alma Pramac Racing) then fell at the end of the session after a slight collision with Bradley Smith (Red Bull KTM Factory Racing) at Turn 5 – rider ok.